| Bactericidal activity of immobilized silver nanoparticles on silica substrates with different sizes | |
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| 년도 | 2021 |
| 날짜 | Nov |
| 페이지 / 학회지명 |
doi: 10.1007/s11356-021-17710-0 / Environmental Science and Pollution Research |
| 논문저자 | Young-Seon Ko 1 2, SungJun Park 3 4, GwangPyo Ko 5 6 7, Kyoungja Woo 8 |
| Link |
https://link.springer.com/article/10.1007/s11356-021-17710-0
115회 연결
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1 Nanophotonics Research Center, Korea Institute of Science and Technology, 5, Hwarang-ro 14-gil, Seongbuk-gu, Seoul, 02792, Republic of Korea. 2 Research Institute of Industrial Science & Technology (RIST), Pohang, Republic of Korea. 3 Department of Environmental Health Sciences, Graduate School of Public Health, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, Republic of Korea. 4 N-Bio, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, Republic of Korea. 5 Department of Environmental Health Sciences, Graduate School of Public Health, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, Republic of Korea. gko@snu.ac.kr. 6 N-Bio, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, Republic of Korea. gko@snu.ac.kr. 7 Institute of Health and Environment, Seoul National University, Seoul, Republic of Korea. gko@snu.ac.kr. 8 Nanophotonics Research Center, Korea Institute of Science and Technology, 5, Hwarang-ro 14-gil, Seongbuk-gu, Seoul, 02792, Republic of Korea. kjwoo@kist.re.kr. Abstract Hybrid particles with immobilized silver nanoparticles (AgNPs) receive a lot of attention due to their excellent antibacterial activity with the prevention of inherent aggregation of AgNPs. In this study, serial sized silica substrate particles (231, 401, and 605 nm) and their corresponding hybrid particles with AgNPs (~ 30 nm) are prepared, with detailed bactericidal images of the corresponding particles at various times. Their bactericidal activity is elucidated for both Gram-positive Streptococcus agalactiae and Gram-negative Escherichia coli CN13, which show the size of 0.8 μm × 0.9 μm and 1.3 μm × 1.8 μm, respectively. There is a large difference in the bactericidal activity between the smallest (231 nm, 3-log10 reduction) and larger (401 and 605 nm, 6-log10 reduction) silica substrates, whereas there is hardly a difference between the latter. Their effective total surface area (ETSA) is considered important for their bactericidal activity, based on the nearly equal large ETSA of the well-dispersed two larger silica substrates and the much smaller ETSA of the agglomerated smallest substrates. Submicron-sized pits appear on the bacterial membrane by direct contact with the hybrid particles, implicating the importance of ETSA. Still, further research is needed with much different silica substrate sizes to fully elucidate the impact of the silica substrate on the bactericidal activity of immobilized AgNPs. Keywords: AgNP@SiO2; Bacterial membrane; Direct contact; Effective total surface area (ETSA); Hybrid particle; Pit. |
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