| Antiviral Effects of Lindera obtusiloba Leaf Extract on Murine Norovirus-1 (MNV-1), a Human Norovirus Surrogate, and Potential Application to Model Foods | |
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| 년도 | 2020 |
| 날짜 | 2020 Oct 14 |
| 페이지 / 학회지명 |
9(10):697 / Antibiotics |
| 논문저자 | Diana Solis-Sanchez 1, Adriana Rivera-Piza 1, Soyoung Lee 1, Jia Kim 1, Bomi Kim 1, Joo Bong Choi 1, Ye Won Kim 1, Gwang Pyo Ko 2, Moon Jung Song 3, Sung-Joon Lee 1 |
| Link |
https://www.mdpi.com/2079-6382/9/10/697
86회 연결
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1 Department of Biotechnology, School of Life Sciences and Biotechnology for BK21 PLUS, Korea University, Seoul 02841, Korea. 2 Institute of Health and Environment, Department of Environmental Health, Center for Human and Environmental Microbiome, Graduate School of Public Health, Seoul National University, Seoul 151-742, Korea. 3 Virus-Host Interactions Laboratory, Department of Biosystems and Biotechnology, Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Korea. Abstract Noroviruses are the leading cause of acute gastroenteritis and food poisoning worldwide. In this study, we investigated the anti-noroviral activity of Lindera obtusiloba leaf extract (LOLE) using murine norovirus (MNV-1), a surrogate of human norovirus. Preincubation of MNV-1 with LOLE at 4, 8, or 12 mg/mL for 1 h at 25 °C significantly reduced viral infectivity, by 51.8%, 64.1%, and 71.2%, respectively. Among LOLE single compounds, β-pinene (49.7%), α-phellandrene (26.2%), and (+)-limonene (17.0%) demonstrated significant inhibitory effects on viral infectivity after pretreatment with MNV-1, suggesting that the anti-noroviral effects of LOLE may be due to the synergetic activity of several compounds, with β-pinene as a key molecule. The inhibitory effect of LOLE was tested on the edible surfaces of lettuce, cabbage, and oysters, as well as on stainless steel. After one hour of incubation at 25°C, LOLE (12 mg/mL) pretreatment significantly reduced MNV-1 plaque formation on lettuce (76.4%), cabbage (60.0%), oyster (38.2%), and stainless-steel (62.8%). These results suggest that LOLE effectively inhibits norovirus on food and metal surfaces. In summary, LOLE, including β-pinene, may inactivate norovirus and could be used as a natural agent promoting food safety and hygiene. Keywords: Lindera obtusiloba leaf extract; MNV-1; anti-noroviral activity; β-pinene. |
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