| Clostridium fessum sp. Nov., isolated from human faeces | |
|---|---|
| 년도 | 2021 |
| 날짜 | 2021 Jan |
| 페이지 / 학회지명 |
71(1) / International Journal of Systematic and Evolutionary Microbiology |
| 논문저자 | Boram Seo 1 2, Kyungchan Jeon 2, Inwoo Baek 3, Yung Mi Lee 4, Kiwoon Baek 5, GwangPyo Ko 6 2 7 8 |
| Link |
https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs…
108회 연결
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2 Department of Environmental Health Sciences, Graduate School of Public Health, Seoul National University, Seoul, Republic of Korea. 3 Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Republic of Korea. 4 Division of Polar Life Sciences, Korea Polar Research Institute, Incheon, Republic of Korea. 5 Bioresources Collection & Bioinformation Department, Nakdonggang National Institute of Biological Resources, Sangju, Republic of Korea. 6 Center for Human and Environmental Microbiome, Institute of Health and Environment, Seoul National University, Seoul, Republic of Korea. 7 KoBioLabs, Inc., Seoul, Republic of Korea. 8 Bio-MAX/N-Bio, Seoul National University, Seoul, Republic of Korea. Abstract An obligately anaerobic, Gram-stain-positive and spore-forming strain, SNUG30386T was isolated from a faecal sample of a healthy Korean subject. The strain formed a round ivory-coloured colony and cells were chained rods with tapered ends, approximately 2.0-2.5×0.6-0.8 μm in size. The taxonomic analysis indicated that strain SNUG30386T was within the family Lachnospiraceae. According to the 16S rRNA gene sequence similarity, the closest species to strain SNUG30386T was Clostridium symbiosum (95.6 %), followed by Enterocloster asparagiformis (94.8 %), Enterocloster clostridioformis (94.8 %) and Enterocloster lavalensis (94.6 %). The evolutionary tree based on 16S rRNA gene sequences demonstrated that strain SNUG30386T had split apart at a unique branch point far from other close relatives. Its DNA G+C content was 48.3 mol% calculated from the whole genome sequence. The major cellular fatty acids were C16 : 0 and C14 : 0. Compared to those of the closely related species, strain SNUG30386T showed distinct biochemical activities such as being unable to utilize most of carbon sources except d-glucose and l-arabinose. As a result, based on its unique phylogenetic clade and taxonomic characteristics, we conclude that strain SNUG30386T represents a novel species within the genus Clostridium, for which the name Clostridium fessum sp. nov. is proposed. The type strain of the novel species is SNUG30386T (=KCTC 15633T= JCM 32258T). Keywords: Clostridium cluster XIVa; Lachnospiraceae; gut microbiome. |
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