Abstract
Hybrid particles with immobilized silver nanoparticles (AgNPs) receive a lot of attention due to their excellent antibacterial activity with the prevention of inherent aggregation of AgNPs. In this study, serial sized silica substrate particles (231, 401, and 605 nm) and their corresponding hybrid particles with AgNPs (~ 30 nm) are prepared, with detailed bactericidal images of the corresponding particles at various times. Their bactericidal activity is elucidated for both Gram-positive Streptococcus agalactiae and Gram-negative Escherichia coli CN13, which show the size of 0.8 μm × 0.9 μm and 1.3 μm × 1.8 μm, respectively. There is a large difference in the bactericidal activity between the smallest (231 nm, 3-log10 reduction) and larger (401 and 605 nm, 6-log10 reduction) silica substrates, whereas there is hardly a difference between the latter. Their effective total surface area (ETSA) is considered important for their bactericidal activity, based on the nearly equal large ETSA of the well-dispersed two larger silica substrates and the much smaller ETSA of the agglomerated smallest substrates. Submicron-sized pits appear on the bacterial membrane by direct contact with the hybrid particles, implicating the importance of ETSA. Still, further research is needed with much different silica substrate sizes to fully elucidate the impact of the silica substrate on the bactericidal activity of immobilized AgNPs.
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Funding
This work was supported by KIST (Project No. 2E30120) and a National Research Foundation of Korea (NRF) (Grant No. 2014–060222).
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Y-SK: Data curation, Formal analysis, Investigation, Visualization, Writing – Original draft, Visualization. SP: Data curation, Investigation, Visualization, Writing – Original draft. GK: Methodology, Supervision, Writing – review & editing. KW: Funding acquisition, Methodology, Project administration, Supervision, Writing – review & editing.
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Young-Seon Ko and SungJun Park have contributed equally to this work
Highlights
• Various sized AgNP@SiO2 particles (0.2, 0.4 and 0.6 µm) can be prepared.
• Two larger particles exhibit much higher bactericidal effects.
• AgNP@SiO2 particles make pits on the bacterial membrane by direct contact.
• Effective surface area of AgNP@SiO2 matters to the bactericidal activity.
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Ko, YS., Park, S., Ko, G. et al. Bactericidal activity of immobilized silver nanoparticles on silica substrates with different sizes. Environ Sci Pollut Res 29, 24180–24190 (2022). https://doi.org/10.1007/s11356-021-17710-0
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DOI: https://doi.org/10.1007/s11356-021-17710-0