| Lactobacillus paracasei treatment modulates mRNA expression in macrophages | |
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| 년도 | 2020 |
| 날짜 | 2020 Jul 21 |
| 페이지 / 학회지명 |
23:100788 / Biochemistry and Biophysics Reports |
| 논문저자 | Dae Hee Han 1, Woon-Ki Kim 1 2, SungJun Park 1 2 3, You Jin Jang 1, GwangPyo Ko 1 2 3 4 5 |
| Link |
https://www.sciencedirect.com/science/article/pii/S2405580820300972?vi…
97회 연결
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1 Graduate School of Public Health, Seoul National University, Seoul, Republic of Korea. 2 N-Bio, Seoul National University, Seoul, Republic of Korea. 3 KoBioLabs, Inc., Seoul, Republic of Korea. 4 Center for Human and Environmental Microbiome, Seoul National University, Seoul, Republic of Korea. 5 Institute of Health and Environment, Seoul National University, Seoul, Republic of Korea. Abstract Macrophage metabolic pathways show changes in response to various external stimuli. Especially, increased lipopolysaccharide, an important bacterial component and Toll-like receptor 4 agonist, can induce activity in various macrophage metabolic pathways, including energy production and biosynthesis, as well as high immune responses due to increase in differentiated M1 macrophages. In this study, we confirmed that Lactobacillus paracasei (L. paracasei) KBL382, KBL384 and KBL385, isolated from the feces of healthy Koreans, can modulate various enzymes and membrane transporters related to glycolysis or macrophage polarization including hypoxia-inducible factor 1-alpha (HIF1A), inducible nitric oxide synthase (iNOS) and arginase in stimulated macrophages at the mRNA level, using the in vitro rodent bone-marrow-derived macrophage (BMDM) model. All L. paracasei exhibited significant down-regulatory effects on mRNAs for glycolysis-related enzymes, including lactate dehydrogenase A, solute carrier family 2 member 1, and triosephosphate isomerase. Moreover, L. paracasei treatment could lead to significant reductions in HIF1A or iNOS mRNA, and induced arginase mRNA in the BMDM model. Therefore, further extensive studies should be performed to support the application of L. paracasei, such as in probiotics or therapeutics, in controlling abnormal immune responses related to macrophage. Keywords: Glycolysis; Immunomodulation; Lactobacillus paracasei; Macrophage; Macrophage polarization; Probiotics. |
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